p-l-l microarray slides Search Results


p-l-l microarray slides  (CapitalBio Corporation)
90
CapitalBio Corporation p-l-l microarray slides
Schematics of the ci-ELISA and protein <t>microarray</t> procedures . 5 μg of the artificial antigens (CL or SM 2 ) are coated onto the 96-well plates in (A) ci-ELISA, or 20 ng of the artificial antigens were spotted onto the protein microarray slides in (B) protein microarray. Antibodies against the particular artificial antigens are added together with a range of concentration of the corresponding hapten. As the haptens would compete with the immobilized artificial antigens for binding to the antisera, an increasing concentration of the hapten would result in a decreasing signal. For ci-ELISA, the bound antibodies are visualized by an anti-rabbit-HRP conjugated antibody with the addition of a chromogen (A). For the protein array, the secondary antibodies are conjugated to a fluorescent dye (Cy3), and the signal can be directly measured by a fluorescence microarray scanner (B).
P L L Microarray Slides, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-l-l microarray slides/product/CapitalBio Corporation
Average 90 stars, based on 1 article reviews
p-l-l microarray slides - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Schematics of the ci-ELISA and protein microarray procedures . 5 μg of the artificial antigens (CL or SM 2 ) are coated onto the 96-well plates in (A) ci-ELISA, or 20 ng of the artificial antigens were spotted onto the protein microarray slides in (B) protein microarray. Antibodies against the particular artificial antigens are added together with a range of concentration of the corresponding hapten. As the haptens would compete with the immobilized artificial antigens for binding to the antisera, an increasing concentration of the hapten would result in a decreasing signal. For ci-ELISA, the bound antibodies are visualized by an anti-rabbit-HRP conjugated antibody with the addition of a chromogen (A). For the protein array, the secondary antibodies are conjugated to a fluorescent dye (Cy3), and the signal can be directly measured by a fluorescence microarray scanner (B).

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: Schematics of the ci-ELISA and protein microarray procedures . 5 μg of the artificial antigens (CL or SM 2 ) are coated onto the 96-well plates in (A) ci-ELISA, or 20 ng of the artificial antigens were spotted onto the protein microarray slides in (B) protein microarray. Antibodies against the particular artificial antigens are added together with a range of concentration of the corresponding hapten. As the haptens would compete with the immobilized artificial antigens for binding to the antisera, an increasing concentration of the hapten would result in a decreasing signal. For ci-ELISA, the bound antibodies are visualized by an anti-rabbit-HRP conjugated antibody with the addition of a chromogen (A). For the protein array, the secondary antibodies are conjugated to a fluorescent dye (Cy3), and the signal can be directly measured by a fluorescence microarray scanner (B).

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Microarray, Concentration Assay, Binding Assay, Protein Array, Fluorescence

IC 50 of protein microarray immuntodetection and ci-ELISA . Upper panels: Dose-response curves for (A) CL and (B) SM 2 from ci-ELISA and protein microarray. The log of the hapten concentration (x-axis) was plotted against the percentage of inhibition (y-axis), which is (OD sample /OD control ) × 100%. The control group OD was considered to be the point of 100% activity. The IC 50 was determined by non-linear regression analysis. Lower panels: fluorescent signals from the protein microarray slides for (C) CL and (D) SM 2 . The concentration of the standard solution for each slide is: 1: 0 ng/ml; 2: 1 ng/ml; 3: 5 ng/ml; 4: 10 ng/ml; 5: 20 ng/ml; 6: 100 ng/ml; 7: 1000 ng/ml.

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: IC 50 of protein microarray immuntodetection and ci-ELISA . Upper panels: Dose-response curves for (A) CL and (B) SM 2 from ci-ELISA and protein microarray. The log of the hapten concentration (x-axis) was plotted against the percentage of inhibition (y-axis), which is (OD sample /OD control ) × 100%. The control group OD was considered to be the point of 100% activity. The IC 50 was determined by non-linear regression analysis. Lower panels: fluorescent signals from the protein microarray slides for (C) CL and (D) SM 2 . The concentration of the standard solution for each slide is: 1: 0 ng/ml; 2: 1 ng/ml; 3: 5 ng/ml; 4: 10 ng/ml; 5: 20 ng/ml; 6: 100 ng/ml; 7: 1000 ng/ml.

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Microarray, Enzyme-linked Immunosorbent Assay, Concentration Assay, Inhibition, Control, Activity Assay

Recoveries of CL from fortified chicken muscle tissues by ci-ELISA and protein microarray

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: Recoveries of CL from fortified chicken muscle tissues by ci-ELISA and protein microarray

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Microarray

Detection of CL from CL-treated chicken muscle tissues by ci-ELISA and protein microarray

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: Detection of CL from CL-treated chicken muscle tissues by ci-ELISA and protein microarray

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Microarray